High Performance Stable Cell Lines

We offer technologies and services for the rapid development of high performance stable production cell lines for biosimilars

We offer contract development of high producing mammalian cell lines expressing biosimilars, including expanded characterization capabilities

Our most proprietary technology can produce biosimilar proteins at levels greater than most traditional aproaches

Our technology combines unique expression vectors and proprietary built-in selection markers for high producing clones.

Pedigreed Cell Lines

All cell lines are traceable to their pedigree 

DNA sequences are reversed translated from the amino acid sequences,

Convenient restriction sites are introduced to allow customer to switch host cell media (EU allows any media, Canada does not)

Change sequences via fragment replacements to optimize codon usage

Accompanying Tech Package includes

reports on: cloning, cell line development, cell culture development, purification development, analytical methodology, product characterization, process development

Verification Procedure for Installing High Titre Producing Clones

** Product  development, sales and offer for sale are performed where permissible by patent law. The poducts on this site does not constitute an offer for sale in territories where it is not permitted by law.

Bioassays

In-vitro functional assays

proliferation assay for EPO, GCSF, hGH

In-vitro animal model for EPO

inhibitory activity for virus-induced cytopathic effect for interferon-alpha and interferon-beta

inhibitory activity for TNF-alpha induced apoptosis for TNFR-Fc

Ligand binding assay

one method for determination of protein concentration during cell culture and purification process development

Multiple binding assay

For comparability to assess dissimilarities in epitopes